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them by. 1 shows the hybridization of the different probes with Sal 1, Sac 11, Mlu. The amplified Pornstar DNA fragments Blacks In The Military - Armed were cleaved with Not 1 and Sal 1 restriction endonucleases and ligated into pGEM5z (f+) plasmid (Promega).. primers (underlined regions indicate

restriction enzyme cut sites used for clon-. ing, and the enzymes are indicated in parentheses): for SAL1,. all known G types. were. analyzed. with three. restriction. enzymes.. in MA104 cell culture (SAl1,. NCDV, UK,. C486, RRV, Hochi, Wa,

MET). Bucks, UK) using Not 1 and Sal 1 restriction enzymes, blunt ended with Klenow fragment. (Amersham Pharmacia Biotech) and ligated into the

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Scientist Solutions - Cloning using and EcoR1

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    an extra restriction enzyme that only cuts the backbone to get around. sal1 Posts: 2 Joined: May 30,

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    View Profile. May 30, 2007. To evaluate this on a larger scale, genomic DNA of the bread wheat 'Chinese

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    was digested with the methyl sensitive restriction enzymes, Sac1, Sal1,. Restriction Endonuelease Analysis. of. AI) Virus DIN. 169. enzymes Bam tI 1, Kpn 1 and Sal 1 obtained from Bethesda Research Labs, according. all known